Antibodies to 18 nuclear and cytoplasmic antigens IgG (dsDNA, Nucleosomes, Sm, Sm/RNP, P0, Histons, RNP, SS-A/Ro60, SS-A/Ro52, SS-B/La, Scl-70, PmScl100, CENP-B, PCNA, Jo-1, AMA-M2, DFS 70,f-actin), immunoblot.